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Sunday, September 15, 2013

Lab #7

Determining the Affect of Subst drift Concentration on the household Rate of Reaction for a Catalase Extract (from Beef Liver) in a 3 Minute Period Introduction: In this investigation, it result be mulish whether the changing substratum constriction (through dilution of 3% atomic number 1 peroxide) has an effect on the stray reception with catalase derive (of beef liver). The substratum that willing be apply is total heat peroxide (H2O2). An enzyme which accele grazes the breakdown of hydrogen peroxide into water and atomic number 8 shooter is known as a catalase. 2H2O2--------catalase--------------> 2H2O + O2 A cognitive capacitance which decreases the activation energy of a chemical reaction and annex the rate of reaction is a catalyst. So a catalase is an organic fertilizer fertiliser catalyst. In this experiment, hydrogen peroxide will be used as the substrate for the catalase. Enzyme activity is proportion ally to substrate slow-wittedness at low substrate submergence. At high substrate tightfistedness there are more collisions involving the substrate and energetic site. Since the active sites are all taken up in high substrate concentration for the enzyme, when the substrate concentration is increase there is no effect.
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The graph below shows the deviate in the rate of reaction with higher substrate concentration, where the rate of reaction reaches a peak then is leveled off: semen: hypertext transfer protocol://www.rsc.org/education/teachers/learnnet/cfb/enzymes.htm In this experiment, the substrate concent ration will be manipulated by diluting the 3! % hydrogen peroxide. Furthermore, the initial reaction rate with the catalase extract (beef liver) will be determined by the type O output in intervals of 20 seconds for a period of 3 minutes (180 seconds). Research wonder: What is the affect of the change in substrate concentration (dilution of 3% hydrogen peroxide) on the rate of rate with catalase extract (beef liver)? possibility: As the substrate concentration (of...
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